The rollout follows three years of store development and product trials at its flagship Lincoln store.
Where possible each 3,000sq ft store will use local ingredients for its food-to-go range of sandwiches, wraps, salads, cakes and meals.
Foo Go founder George Robinson said the company had earmarked 40 sites in market towns and cathedral cities where there was a greater connection with the local rural economy.
The company's positive relationship with its suppliers had helped fuel the success of its Lincoln store, he said. "We know all of our suppliers, but our customers know many of them too, which helps reinforce our positioning."
Foo Go already supplies a range of on-the-go products in dedicated chiller units to more than 130 retail outlets.